![]() ![]() We describe a recombinant inbred line (RIL) population of 161 F5 genotypes for the widest euploid cross that can be made to cultivated sorghum (Sorghum bicolor) using conventional techniques, S. Publication Date: Research Org.: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States) Sponsoring Org.: USDOE Office of Science (SC), Biological and Environmental Research (BER) National Science Foundation (NSF) OSTI Identifier: 1616088 Grant/Contract Number: AC02-05CH11231 Resource Type: Accepted Manuscript Journal Name: Scientific Reports Additional Journal Information: Journal Volume: 9 Journal Issue: 1 Journal ID: ISSN 2045-2322 Publisher: Nature Publishing Group Country of Publication: United States Language: English Subject: 59 BASIC BIOLOGICAL = , for Biotechnology, Huntsville, AL (United States) USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States) HudsonAlpha Inst.of Georgia, Athens, GA (United States) Monsanto Company, Huxley, IA (United States) The maps will provide a valuable tool for plant breeders and others in the Paspalum community to identify traits of interest, including salt tolerance. These high-density maps are the first available genetic maps for seashore paspalum. Four inversions were identified, two of which were sorghum-specific while the other two were likely specific to seashore paspalum. Comparative analyses showed that each seashore paspalum chromosome was syntenic to and highly colinear with a single sorghum chromosome. Markers formed 10 linkage groups, corresponding to the ten seashore paspalum chromosomes. The AH, HA and HH maps each comprised more than 1000 markers. We also generated maps that consisted of markers that were heterozygous in both parents (HH maps). More markers were identified that segregated in the maternal parent (HA maps) compared to the paternal parent (AH maps), suggesting that 509022 had overall higher levels of heterozygosity than HI33. Progeny were genotyped using a genotyping-by-sequencing (GBS) approach and sequence reads were analyzed for single nucleotide polymorphisms (SNPs) using the UGbS-Flex pipeline. As a step towards trait mapping in the halophyte seashore paspalum (Paspalum vaginatum Sw.), we developed an F 1 mapping population from a cross between two genetically diverse and heterozygous accessions, 509022 and HI33. ![]()
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